Reports the related metrics for all eight RNA-seq samples, including library sizes, the mapping summaries, and the counting summaries. Tables S2. and S3. Tabulate the overlapping summaries of Gencode V19 annotation database at both the gene and the nucleotide base levels, respectively. Figures S1. and S2. Show all-against-all scatter plots of gene expression profile among RNA-seq samples sequenced by stranded and non-stranded protocols, respectively. Figure S3. Explains why the expression level for GAPDH (a well-known housekeeping gene) is underestimated in non-stranded RNA-seq. Script 1. Contains the R script to estimate the gene overlap in Gencode Release 19. (PDF 429 kb
Intersections of detected genes among tested pipelines. Genes with TPM >â0.1 were defined as being...
Figure S1. MA plots showing the differentially expressed transcripts detected by Trad-KAPA and 3â-...
Impact of gene length correction on correlation. Simulated expression data of 10 genes in 2 samples....
Table S1. Sequencing and mapping statistics. Listed are the sequencing details and mapping statistic...
Gene coverage calculation (Table S1), reciprocal best BLAST paring (Table S2), full annotation and R...
Impact of gene lengths and expression levels on gene quantification correlations among pipelines. (a...
Genes with at least two-fold change in expression between UHRR and HBRR have a nearly even distribut...
Differential expression analysis of protein-coding genes. (a) Comparison of measured fold-changes be...
An illustration of various macro-molecules interacting with the mRNA transcript and regulatory signa...
Venn diagrams of the DEGs comparing 3 biological replicates from the SE data with 2 biological repli...
Table S1. Not1-site-containing transcripts in mouse. Table S2. Not1-site-containing transcripts in h...
RNA-Seq data sets. List of Sequence Read Archive accession numbers of all included RNA-Seq data sets...
Additional file 2: Table S1. Coverage uniformity across gene body and gene number detected by SHERRY...
Vignette on the comparison based on expression analysis. Figure S1 to S9. Experiment design and cont...
Influence of multi-hits on isoform and gene expression levels. Comparison of the expression estimate...
Intersections of detected genes among tested pipelines. Genes with TPM >â0.1 were defined as being...
Figure S1. MA plots showing the differentially expressed transcripts detected by Trad-KAPA and 3â-...
Impact of gene length correction on correlation. Simulated expression data of 10 genes in 2 samples....
Table S1. Sequencing and mapping statistics. Listed are the sequencing details and mapping statistic...
Gene coverage calculation (Table S1), reciprocal best BLAST paring (Table S2), full annotation and R...
Impact of gene lengths and expression levels on gene quantification correlations among pipelines. (a...
Genes with at least two-fold change in expression between UHRR and HBRR have a nearly even distribut...
Differential expression analysis of protein-coding genes. (a) Comparison of measured fold-changes be...
An illustration of various macro-molecules interacting with the mRNA transcript and regulatory signa...
Venn diagrams of the DEGs comparing 3 biological replicates from the SE data with 2 biological repli...
Table S1. Not1-site-containing transcripts in mouse. Table S2. Not1-site-containing transcripts in h...
RNA-Seq data sets. List of Sequence Read Archive accession numbers of all included RNA-Seq data sets...
Additional file 2: Table S1. Coverage uniformity across gene body and gene number detected by SHERRY...
Vignette on the comparison based on expression analysis. Figure S1 to S9. Experiment design and cont...
Influence of multi-hits on isoform and gene expression levels. Comparison of the expression estimate...
Intersections of detected genes among tested pipelines. Genes with TPM >â0.1 were defined as being...
Figure S1. MA plots showing the differentially expressed transcripts detected by Trad-KAPA and 3â-...
Impact of gene length correction on correlation. Simulated expression data of 10 genes in 2 samples....