Alba-domain proteins translocate from the cytoplasm to the flagellum and the nucleolus upon <i>Leishmania</i> amastigote differentiation.

<p>Subcellular localization of <i>Li</i>Alba1-HA and HA-<i>Li</i>Alba3 proteins in promastigotes (A) and upon amastigote differentiation (8 h in MAA medium pH 5.8 at 37°C) (B) was assessed by indirect immunofluorescence studies using an anti-HA antibody as described in Materials and Methods. DAPI staining (red) allows detection of the nucleus (N) and kinetoplastid DNA (K). C) Immunofluorescence images of wild type <i>L</i>. <i>infantum</i> episomally co-expressing pSP-NEOalphaIR-eYPF-<i>Li</i>Alba1 and pSP-HYGalphaIR-mCh-<i>Li</i>NOP10 grown as promastigotes (Pro), differentiating amastigotes (Diff) and amastigotes (Ama). <i>Li</i>NOP10 was used as a nucleolar (Nu) control.