Genetic interactions between Clathrin-dependent endocytosis, and endocytic trafficking genes, and <i>slit</i> and <i>robo</i>.

<p>(A) An ipsilateral subset of axons in the ventral nerve cord of <i>WT</i> stage 16 <i>Drosophila</i> embryos are stained with a monoclonal antibody (mAb) to FasciclinII (FasII), and quantified in the histogram below as having 0% error in the number of embryonic segments with fascicles crossing the midline. (B) Double heterozygous <i>slit</i>, <i>robo</i> embryos have a mild loss-of-repulsion phenotype (induction of ectopic crossing events in 16% of embryonic segments). Inhibiting Clathrin-dependent endocytosis by removing one copy of either <i>α-adaptin</i> or <i>endophilinA</i> in the <i>slit</i>,<i>robo/+</i> background enhances the number of crossing defects (C, D), as does inhibiting either entry into the early endosome by removing one copy of <i>rab5</i> (E), or entry into the late endosome by removing one copy of <i>rab7</i> (F). These genetic enhancements of the <i>slit</i>,<i>robo/+</i>, but not of the <i>+/+</i>, ectopic crossing frequency are statistically significant (*, indicates p<0.0001) by two-way ANOVA, Sidak’s 95% Confidence Interval. Error bars indicate standard error of the mean. (<i>+/+</i> n (number of segments) = 121, <i>slit</i>^<i>1</i>,<i>robo</i>^<i>5</i>/+: 132; <i>α-ada</i>^<i>1</i>/+ 121, <i>α-ada</i>^<i>1</i>/ <i>slit</i>^<i>1</i>,<i>robo</i>^<i>5</i> 99; <i>α-ada</i>^<i>3</i>/+ 121, <i>α-ada</i>^<i>3</i>/ <i>slit</i>^<i>1</i>,<i>robo</i>^<i>5</i> 154; endoA^<i>10</i>/+ 121, <i>slit</i>^<i>1</i>,<i>robo</i>^<i>5</i>/+; endoA^<i>10</i>/+ 154; endoA^<i>EP927</i>/+ 121, <i>slit</i>^<i>1</i>,<i>robo</i>^<i>5</i>/+; endoA^<i>EP927</i>/+ 121; <i>rab5</i>^{<i>k08232</i>}/+ 121, <i>slit</i>^<i>1</i>,<i>robo</i>^<i>5</i>/<i>rab5</i>^{<i>k08232</i>} 154; <i>rab5</i>^<i>2</i>/+ 121, <i>slit</i>^<i>1</i>,<i>robo</i>^<i>5</i>/<i>rab5</i>^<i>2</i> 121; rab7^{<i>EY10675</i>}/+ 121, <i>slit</i>^<i>1</i>,<i>robo</i>^<i>5</i>/+; rab7^{<i>EY10675</i>}/+ 176; rab7^{<i>FRT82B</i>}/+ 121, <i>slit</i>^<i>1</i>,<i>robo</i>^<i>5</i>/+; rab7^{<i>FRT82B</i>}/+ 154.)