A-B)Examples of double staining immunohistochemistry to identify M1 and M2 TAMs in cervical cancer patients. Expression of CD68 is indicated by brown cytoplasmic/membranous staining. The expression of transcription factors pSTAT and c-MAF is indicated by red nuclear staining. Representative examples have been presented of cervical cancer patients with high numbers of M1 (CD68+pSTAT1+) cells (A; arrows indicate M1 cells), and M2 (CD68+c-MAF+) cells (B; arrows indicate M2 cells) (original magnification 200X; insert magnification 1000X). C) Overall survival curves in LACC patients with high and low M1 levels (solid line = high M1, dashed line = low M1). D) Overall survival curves in LACC patients with high and low M2 levels (solid line = high M2, dashed line = low M2).

<p>A-B)Examples of double staining immunohistochemistry to identify M1 and M2 TAMs in cervical cancer patients. Expression of CD68 is indicated by brown cytoplasmic/membranous staining. The expression of transcription factors pSTAT and c-MAF is indicated by red nuclear staining. Representative examples have been presented of cervical cancer patients with high numbers of M1 (CD68+pSTAT1+) cells (A; arrows indicate M1 cells), and M2 (CD68+c-MAF+) cells (B; arrows indicate M2 cells) (original magnification 200X; insert magnification 1000X). C) Overall survival curves in LACC patients with high and low M1 levels (solid line = high M1, dashed line = low M1). D) Overall survival curves in LACC patients with high and low M2 levels (solid line = high M2, dashed line = low M2).