KPT-185 diminishes HSP70, HSF1 and p-HSF1^Ser326 expression.

<p>After an 18-h treatment of KPT-185 (i.e., 50 nM for Z138, 100 nM for JVM2, MINO, and Jeko-1), the cells were lysed and analyzed HSP70 (A) and HSF1 and p-HSF1Ser326 expression (B) by immunoblot as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0137210#pone.0137210.g002" target="_blank">Fig 2</a>. α-tubulin was used as a loading control. The intensity, compared to that of α-tubulin or p-HSF1 / HSF1 levels after background subtraction were obtained using ImageJ software. The results are representative of three independent experiments.α.