ACh concentration-response for most of the functional mutants.

<p><b>A</b>. Representative current traces induced by ACh for the wild type and mutant receptors with the concentrations indicated. <b>B</b>. Averaged and normalized concentration-responses of charge (6–8 oocytes each group). Lines are nonlinear least squares fits of the normalized averages of the responses to the Hill equation. <b>C</b>. Bar graph of the pEC50 values (negative logEC₅₀s) derived from B. ***: P<0.001 when compared to the WT value.