Up-regulation of CDKN1A (p21^Waf1/Cip1) was observed in INO80 complex knockdown HeLa or 293T cells.

<p>(<b>A</b>) Verification of the mRNA of select genes from gene expression profiles. HeLa cells were transfected with 15pmol INO80- or hArp8-siRNA and siNT (as control). 48 hours after transfection, cells were harvested, and select genes from gene expression profiles were assessed by RT-qPCR. Bar graphs show ratios of RT-qPCR signals to GAPDH (all signals normalized to siNT). Error bars represent the standard error of the mean of 3 independent experiments. (<b>B</b>) Elevation of p21 protein expression in INO80- or hArp8-siRNA knockdown 293T cells. Cells were treated with indicated siRNA. 48 hours later, proteins were detected with WB using indicated antibodies. (<b>C</b>) High expression of p21 protein in cells as assessed by immunofluorescence. INO80- or hArp8-siRNA (15 or 30pmol) and siNT control was transfected into HeLa cells. 48 hours after transfection, immunofluorescence staining was performed. DAPI staining shows total nuclei. p21-positive cells were counted, and the percentage in the total cell numbers was represented in bar graph (<b>D</b>). Error bars represent the standard error of the mean of 2 independent experiments. **<i>p</i> < 0.01 in comparison with siNT control (Student <i>t</i> test).