Root mean squared displacement maps of the 13–16 somite embryo registration pipelines.

<p>When a sagittal slice through the average autofluorescence image of the 13–16 somite <i>Dll4</i> population average (A) or the average vasculature image (A′) is overlaid with the root mean squared (RMS) displacement map generated in the registration pipeline, the movement of voxels required for proper alignment of the embryos can be visualized and quantified (B) (n = 14). The heat map scale bar (left side of B) indicates the voxel displacement in μm. The same is shown, respectively, for the 13–16 somite <i>Mlc2a</i> embryos (C, C′, and D), and the <i>Eng</i> embryos (E, E′, and F; n = 14). Scale bar = 500 μm.