Registration of mutant mice across all three mouse lines reveals that <i>Dll4</i> have significantly different Jacobian determinant maps compared to <i>Mlc2a</i> and <i>Eng</i> mice.

<p><i>Dll4</i>^-/- mice were compared to the pooled population of <i>Mlc2a</i>^-/- and <i>Eng</i>^-/- mice at all time points after registration. (A-C) are sagittal slices through the average 17–20 somite autofluorescence image of all the mutants in the pipeline overlaid with the Jacobian determinant heat map generated during the registration, and in each slice it is evident that the <i>Dll4</i> mice are smaller than the other mutants in both the trunk and heart regions (blue shading). This is also qualitatively obvious when isosurface images of representative <i>Eng</i>^-/- (D), <i>Mlc2a</i>^-/- (E), and <i>Dll4</i>^-/- mice are shown side-by-side. The <i>Dll4</i>^-/- mouse also has a significantly growth-delayed, linear heart tube (compare heart tube geometry between D-F). The colour bar presents the t-statistic of which the minimum corresponds to a FDR threshold of 10%. Scale bar = 500μm.