LNFPIII induces Raf-1 kinase activation <i>via</i> TLR4 and CD14 receptors.

<p>a) BMDCs from <i>wt</i> and <i>Tlr4</i>^<i>-/-</i> mice were stimulated with LPS (100ng/ml), LNFPIII-Dex (50μg/ml), Dex (50μg/ml) or nothing for 15 mins. Raf-1 kinase activity was carried out as described in materials and methods. The reaction mixtures were fractionated using SDS-PAGE and western blot performed with anti-phospho MBP antibody. b) RAW264.7 macrophages were pre-treated with anti-CD14 antibody and isotype control followed by stimulation with LPS (100ng/ml), LNFPIII-NGC (50μg/ml), Dex (50μg/ml) or nothing for 15 mins, processed for Raf-1 kinase assay and western blot analysis was performed using anti-phospho-MBP antibody.