Characterization of the mRNA levels for selected genes encoding for <i>A</i>. <i>phagocytophilum</i> over-represented proteins.
- Margarita Villar (420749)
- Nieves Ayllón (420744)
- Katherine M. Kocan (326058)
- Elena Bonzón-Kulichenko (792365)
- Pilar Alberdi (420748)
- Edmour F. Blouin (420746)
- Sabine Weisheit (792366)
- Lourdes Mateos-Hernández (525783)
- Alejandro Cabezas-Cruz (420438)
- Lesley Bell-Sakyi (33822)
- Marie Vancová (792367)
- Tomáš Bílý (792368)
- Damien F. Meyer (695399)
- Jan Sterba (792369)
- Marinela Contreras (792370)
- Nataliia Rudenko (792371)
- Libor Grubhoffer (420445)
- Jesús Vázquez (54003)
- José de la Fuente (35632)
Publication date
September 2015
DOIAbstract
<p>The mRNA levels for <i>groEL</i>, <i>msp4</i> and <i>hsp70</i> were determined by real-time RT-PCR in low and high percentage infected ISE6 tick cells. Amplification efficiencies were normalized against tick <i>16S rRNA</i> and mRNA levels expressed in arbitrary units. The ratio of normalized mRNA levels in high to low percentage-infected cells was represented as Ave+SD. Normalized Ct values were compared between low and high percentage infected tick cells by Student's t-test (*P<0.05) (N = 5).</p
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