Glucuronidase assay in human blood and plasma.

<p>(A) GusA was spiked at varying concentrations into assay matrix (PBS with 2% FBS), human plasma and human blood respectively. A glucuronidase assay of the samples was performed using Cl-MUGlcU as substrate. The supernatant of spiked blood was used in the glucuroronidase assay after centrifugation (7 min, 3000 g). Mean plus standard deviation of 4 samples is shown with each concentration of glucuronidase tested in triplicate (n = 12 for each data point). (B) The data for 10⁴ pg/ml glucuronidase are shown in detail. The fluorescence signal derived from the blood sample is significantly higher compared to the signal that was obtained after spiking the same amount of GusA into identical volumes of assay matrix and plasma respectively. No significant difference was observed between the samples of the latter two groups.