Release of dye and drug from liposome is dependent on low pH of acidic organelles.

<p><b>A</b>: Fluorescent microscopy images of MCF7 cells incubated with Doxorubicin-loaded liposomes for 6 hours (red fluorescence). Control cells did not receive liposomes. <b>B</b>: Release of Pyranine dye in MiaPaCa-2 cells is examined by its fluorescence with or without the treatment with Bafilomycin A1. Phase contrast picture is shown. <b>C</b>: Effects of Bafilomycin A1 on pH of intracellular organelles are shown using Acridine Orange.