Effect of MMP-2 activity inhibition on PARP-1 degradation.

<p>The cleaved form of PARP-1 (panel A) was detected in the MMP-2^+/+ livers (lanes 1 and 2) and virtually absent in MMP-2^-/- livers (lanes 3, and 4) at 6h post-IRI; the cleaved PARP/total PARP ratio was profoundly depressed in the MMP-2^-/- livers post-reperfusion. The cleaved PARP/total PARP ratios (panel B) were also markedly decreased in lysates of MMP-2^-/- SECs and MMP-2 inhibitor-treated MMP-2^+/+ SECs. The LDH levels (panel C) were increased in MMP-2^-/- SECs and in MMP-2 inhibitor-treated MMP-2^+/+ SECs, compared with controls (n = 4–5/group; <i>in vitro</i> data is expressed as mean ± SD of four independent experiments; *p<0.05 relative to MMP-2^+/+ samples and **p<0.05 relative to MMP-2^-/- samples).