Phylogenetic analysis of <i>B</i>. <i>pseudomallei</i>, <i>B</i>. <i>mallei</i>, and <i>B</i>. <i>thailandensis</i> strains by <i>bucl</i>-locus typing.

<p>Bayesian analysis was performed on concatenated nucleotide sequences of the non-collagenous regions of <i>bucl3</i>, <i>bucl4</i>, and <i>bucl8</i> present in a set of 13 <i>B</i>. <i>pseudomallei</i>, 11 <i>B</i>. <i>mallei</i>, and 9 <i>B</i>. <i>thailandensis</i> strains (as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0137578#pone.0137578.t003" target="_blank">Table 3</a>). Support values for each branch are shown as posterior probability from Bayesian analysis and bootstrap values from maximum parsimony analysis, respectively (PP/MP). Posterior probability value which was not supported by maximum parsimony analysis is shown in red. Phylogenetic Clusters 1–4 (C1-C4) correlated with geographic location of <i>B</i>. <i>pseudomallei</i> strains, whereas Clusters 5–7 (C5-C7) contained <i>B</i>. <i>thailandensis</i> strains that made up a separate branch from <i>B</i>. <i>pseudomallei</i> and <i>B</i>. <i>mallei</i> strains. Scale bar is representative of evolutionary distance in substitutions per nucleotide.