Primers and probe used for <i>bucl</i> amplification^a.

<p>^a Primers were designed to generate conserved amplicons within the non-collagenous region of each <i>bucl</i> gene. Primers for <i>bucl6</i> and <i>bucl15</i> were designed flanking the collagenous region, which varies in size among strains (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0137578#pone.0137578.s006" target="_blank">S4B Fig</a>). Primers Bucl8_3F/3R were used to generate amplicon from Bp K96243 reference strain, whereas primers Bucl8_2F/2R selectively amplify products from Bp and Bm gDNA and were used in large-scale PCR screening (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0137578#pone.0137578.s006" target="_blank">S4B Fig</a>).</p><p>^b Amplicon sizes expected for the Bp K96243 reference strain.</p><p>^c LNA probe for <i>bucl16</i> detection; + symbols precede LNA bases.</p><p>Primers and probe used for <i>bucl</i> amplification<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0137578#t005fn001" target="_blank">^a</a>.