Heatmap of cellulose regulons and protein profiles of culture supernatants from <i>P</i>. <i>oxalicum</i> strains containing mutations of <i>clrB</i> or/and <i>creA</i> genes.

<p>(A) The hierarchical clustering of expression patterns for the 155 increased genes of cellulose regulon in the mutants versus wild-type strain. Hierarchical clustering analysis was performed using software HCE3.5. Heat maps showing log (RPKM) from minimum (bright green) to maximum (red) revealed four gene sets. (B) SDS-PAGE of proteins from unconcentrated culture supernatants of wild-type strain and <i>clrB</i> or/and <i>creA</i> mutants when cultured on glucose for 22 hours then shifted to cellulose for 96 hours. (C) Venn diagram from analysis of secretomes of culture supernatants from <i>P</i>. <i>oxalicum</i> mutants. The loading samples were normalized to same concentration with buffer.