Results of qPCR for fecal card samples vs. stool/dilution samples for different egg counts.

Comparison of the egg recovery rates and limit of detection for soil-transmitted helminths using the Kato-Katz thick smear, faecal flotation and quantitative real-time PCR in human stool.

Patsy A Zendejas-Heredia
Vito Colella
Sze Fui Hii
Rebecca J Traub

May 2021

BackgroundMonitoring the success of soil-transmitted helminth (STH) control programs relies on accur...

Comparison of the egg recovery rates and limit of detection for soil-transmitted helminths using the Kato-Katz thick smear, faecal flotation and quantitative real-time PCR in human stool

Zendejas-Heredia, PA
Colella, V
Hii, SF
Traub, RJ

May 2021

BACKGROUND: Monitoring the success of soil-transmitted helminth (STH) control programs relies on acc...

Blood collection tube study results comparing qPCR to ddPCR.

Rebecca C. Holmberg (442521)
Alissa Gindlesperger (442522)
Tinsley Stokes (442523)
David Lopez (442524)
Lynn Hyman (442525)
Michelle Freed (442526)
Phil Belgrader (442527)
Jeanne Harvey (442528)
Zheng Li (26302)

August 2013

Correlation plot of percent fetal, % Y (ddPCR vs. qPCR) with a linear fit.</p

Comparison of ELISA and qPCR results.

James T. Nyirenda (16954718)
Marc Y. R. Henrion (9915338)
Vita Nyasulu (16954721)
Mike Msakwiza (16954724)
Wilfred Nedi (6989795)
Herbert Thole (11210932)
Jacob Phulusa (5052161)
Neema Toto (7617830)
Khuzwayo C. Jere (16954727)
Alex Winter (16954730)
Leigh A. Sawyer (16954733)
Thomas Conrad (12994156)
Donnie Hebert (16954736)
Crystal Chen (6535853)
Wesley C. Van Voorhis (10332534)
Eric R. Houpt (8035412)
Pui-Ying Iroh Tam (11210926)
Darwin J. Operario (10198841)

September 2023

Participant stools were monitored for oocyst shedding during the inpatient period and follow-up visi...

Comparison of PCR detection rates in DNA extracted from fecal cards and fresh frozen stool samples.

Mirjam Schunk (794426)
Seleshi Kebede Mekonnen (794427)
Beyene Wondafrash (794428)
Carolin Mengele (276561)
Erna Fleischmann (347957)
Karl-Heinz Herbinger (139085)
Jaco J. Verweij (179271)
Christof Geldmacher (317649)
Gisela Bretzel (139096)
Thomas Löscher (139093)
Ahmed Zeynudin (226298)

September 2015

Comparison of PCR detection rates in DNA extracted from fecal cards and fresh frozen stool sample...

Differences in Ct values by qPCR assay between whole blood (WB) and dried blood spot (DBS) extracted DNA.

Prakash Ghosh (3463907)
Rajashree Chowdhury (8717121)
Md. Abu Rahat (17262440)
Faria Hossain (3463916)
Nur E Arpha (17262443)
Mojca Kristan (6100946)
Matthew Higgins (7633367)
Ahmed Abd El Wahed (9911754)
Yasuyuki Goto (22965)
M. M. Towhidul Islam (10104971)
Susana Campino (56411)
Mary Cameron (3391427)
Malcom S. Duthie (17262446)
Rashidul Haque (60045)
Dinesh Mondal (230634)

October 2023

Differences in Ct values by qPCR assay between whole blood (WB) and dried blood spot (DBS) extracted...

Boxplot of 30 paired PCR-positive samples comparing the distribution of Ct-values in fecal card samples (median Ct-value 33.0) and frozen stool samples (median Ct-value 28.5)

Mirjam Schunk (794426)
Seleshi Kebede Mekonnen (794427)
Beyene Wondafrash (794428)
Carolin Mengele (276561)
Erna Fleischmann (347957)
Karl-Heinz Herbinger (139085)
Jaco J. Verweij (179271)
Christof Geldmacher (317649)
Gisela Bretzel (139096)
Thomas Löscher (139093)
Ahmed Zeynudin (226298)

September 2015

Boxplot of 30 paired PCR-positive samples comparing the distribution of Ct-values in fecal card s...

Comparison of microscopic examination and multiplex qPCR in terms of detection of the major parasites causing gastroenteritis, in stool samples from patients with diarrhea.

Eun Jeong Won (694462)
Soo Hyun Kim (417803)
Seung Jung Kee (3353540)
Jong Hee Shin (694463)
Soon Pal Suh (694469)
Jong Yil Chai (3353543)
Dong Wook Ryang (694470)
Myung Geun Shin (694468)

November 2016

Comparison of microscopic examination and multiplex qPCR in terms of detection of the major paras...

Egg recovery for microscopic validation of qPCR and comparison with the KK and qPCR.

Catherine. A. Gordon (685141)
Luz P. Acosta (167213)
Geoffrey N. Gobert (180959)
Remigio M. Olveda (167220)
Allen G. Ross (263017)
Gail M. Williams (64281)
Darren J. Gray (172051)
Donald Harn (685142)
Yuesheng Li (219117)
Donald P. McManus (172118)

January 2015

Microscopic validation used a modified FEA-SD (Formalin ethyl-acetate sedimentation) technique ap...

Absolute qPCR CT values obtained vs. the genomic equivalence used.

Edwin Kamau (282162)
Saba Alemayehu (451791)
Karla C. Feghali (451792)
David Saunders (221160)
Christian F. Ockenhouse (201973)

August 2013

Data showing the mean CT values obtained from qPCR assays performed using plasmid DNAs...

Results from quantitative PCR (qPCR) on fecal samples and cloacal and fecal float results from experimentally infected and wild caught northern bobwhites (Colinus virginianus).

Whitney M. Kistler (3235047)
Julie A. Parlos (3366101)
Steven T. Peper (633528)
Nicholas R. Dunham (633527)
Ronald J. Kendall (633530)

November 2016

Results from quantitative PCR (qPCR) on fecal samples and cloacal and fecal float results from ex...

Multiplex qPCR Data.

Heather L. Farrington (695021)
Christine E. Edwards (695022)
Xin Guan (6987)
Matthew R. Carr (695023)
Kelly Baerwaldt (695024)
Richard F. Lance (695025)

February 2015

Results of multiplexing trials of qPCR markers. Average Ct values, with the range of copy numbers...

Fecal RT-qPCR results for candidate AIEC transcripts.

Yuanhao Zhang (577563)
Leahana Rowehl (577561)
Julia M. Krumsiek (763107)
Erika P. Orner (763108)
Nurmohammad Shaikh (763109)
Phillip I. Tarr (90158)
Erica Sodergren (48852)
George M. Weinstock (43281)
Edgar C. Boedeker (158288)
Xuejian Xiong (105663)
John Parkinson (4087)
Daniel N. Frank (158306)
Ellen Li (158263)
Grace Gathungu (763110)

June 2015

The number of positive fecal stool samples are shown for each candidate AIEC transcript. Transcri...

Correlation between faecal egg counts and real-time PCR egg estimates.

Nichola Eliza Davies Calvani (4439005)
Peter Andrew Windsor (3129513)
Russell David Bush (4439008)
Jan Šlapeta (77953)

September 2017

(A) Real-time PCR egg estimates (qEPG) for three methods of sample preparation for DNA isolation ...

RT-qPCR results following the extraction-based and homogenization-based protocols represented as Ct values for each patient sample.

Zachary P. Morehouse (3832333)
Lyson Samikwa (7553132)
Caleb M. Proctor (11292285)
Harry Meleke (11292288)
Mercy Kamdolozi (3172389)
Gabriella L. Ryan (11292291)
David Chaima (3515378)
Antonia Ho (10214054)
Rodney J. Nash (11292294)
Tonney S. Nyirenda (11292297)

August 2021

RT-qPCR results following the extraction-based and homogenization-based protocols represented as Ct ...

Comparison of the egg recovery rates and limit of detection for soil-transmitted helminths using the Kato-Katz thick smear, faecal flotation and quantitative real-time PCR in human stool.

Patsy A Zendejas-Heredia
Vito Colella
Sze Fui Hii
Rebecca J Traub

May 2021

BackgroundMonitoring the success of soil-transmitted helminth (STH) control programs relies on accur...

Comparison of the egg recovery rates and limit of detection for soil-transmitted helminths using the Kato-Katz thick smear, faecal flotation and quantitative real-time PCR in human stool

Zendejas-Heredia, PA
Colella, V
Hii, SF
Traub, RJ

May 2021

BACKGROUND: Monitoring the success of soil-transmitted helminth (STH) control programs relies on acc...

Blood collection tube study results comparing qPCR to ddPCR.

Rebecca C. Holmberg (442521)
Alissa Gindlesperger (442522)
Tinsley Stokes (442523)
David Lopez (442524)
Lynn Hyman (442525)
Michelle Freed (442526)
Phil Belgrader (442527)
Jeanne Harvey (442528)
Zheng Li (26302)

August 2013

Correlation plot of percent fetal, % Y (ddPCR vs. qPCR) with a linear fit.</p

Comparison of ELISA and qPCR results.

James T. Nyirenda (16954718)
Marc Y. R. Henrion (9915338)
Vita Nyasulu (16954721)
Mike Msakwiza (16954724)
Wilfred Nedi (6989795)
Herbert Thole (11210932)
Jacob Phulusa (5052161)
Neema Toto (7617830)
Khuzwayo C. Jere (16954727)
Alex Winter (16954730)
Leigh A. Sawyer (16954733)
Thomas Conrad (12994156)
Donnie Hebert (16954736)
Crystal Chen (6535853)
Wesley C. Van Voorhis (10332534)
Eric R. Houpt (8035412)
Pui-Ying Iroh Tam (11210926)
Darwin J. Operario (10198841)

September 2023

Participant stools were monitored for oocyst shedding during the inpatient period and follow-up visi...

Comparison of PCR detection rates in DNA extracted from fecal cards and fresh frozen stool samples.

Mirjam Schunk (794426)
Seleshi Kebede Mekonnen (794427)
Beyene Wondafrash (794428)
Carolin Mengele (276561)
Erna Fleischmann (347957)
Karl-Heinz Herbinger (139085)
Jaco J. Verweij (179271)
Christof Geldmacher (317649)
Gisela Bretzel (139096)
Thomas Löscher (139093)
Ahmed Zeynudin (226298)

September 2015

Comparison of PCR detection rates in DNA extracted from fecal cards and fresh frozen stool sample...

Differences in Ct values by qPCR assay between whole blood (WB) and dried blood spot (DBS) extracted DNA.

Prakash Ghosh (3463907)
Rajashree Chowdhury (8717121)
Md. Abu Rahat (17262440)
Faria Hossain (3463916)
Nur E Arpha (17262443)
Mojca Kristan (6100946)
Matthew Higgins (7633367)
Ahmed Abd El Wahed (9911754)
Yasuyuki Goto (22965)
M. M. Towhidul Islam (10104971)
Susana Campino (56411)
Mary Cameron (3391427)
Malcom S. Duthie (17262446)
Rashidul Haque (60045)
Dinesh Mondal (230634)

October 2023

Differences in Ct values by qPCR assay between whole blood (WB) and dried blood spot (DBS) extracted...

Boxplot of 30 paired PCR-positive samples comparing the distribution of Ct-values in fecal card samples (median Ct-value 33.0) and frozen stool samples (median Ct-value 28.5)

Mirjam Schunk (794426)
Seleshi Kebede Mekonnen (794427)
Beyene Wondafrash (794428)
Carolin Mengele (276561)
Erna Fleischmann (347957)
Karl-Heinz Herbinger (139085)
Jaco J. Verweij (179271)
Christof Geldmacher (317649)
Gisela Bretzel (139096)
Thomas Löscher (139093)
Ahmed Zeynudin (226298)

September 2015

Boxplot of 30 paired PCR-positive samples comparing the distribution of Ct-values in fecal card s...

Comparison of microscopic examination and multiplex qPCR in terms of detection of the major parasites causing gastroenteritis, in stool samples from patients with diarrhea.

Eun Jeong Won (694462)
Soo Hyun Kim (417803)
Seung Jung Kee (3353540)
Jong Hee Shin (694463)
Soon Pal Suh (694469)
Jong Yil Chai (3353543)
Dong Wook Ryang (694470)
Myung Geun Shin (694468)

November 2016

Comparison of microscopic examination and multiplex qPCR in terms of detection of the major paras...

Egg recovery for microscopic validation of qPCR and comparison with the KK and qPCR.

Catherine. A. Gordon (685141)
Luz P. Acosta (167213)
Geoffrey N. Gobert (180959)
Remigio M. Olveda (167220)
Allen G. Ross (263017)
Gail M. Williams (64281)
Darren J. Gray (172051)
Donald Harn (685142)
Yuesheng Li (219117)
Donald P. McManus (172118)

January 2015

Microscopic validation used a modified FEA-SD (Formalin ethyl-acetate sedimentation) technique ap...

Absolute qPCR CT values obtained vs. the genomic equivalence used.

Edwin Kamau (282162)
Saba Alemayehu (451791)
Karla C. Feghali (451792)
David Saunders (221160)
Christian F. Ockenhouse (201973)

August 2013

Data showing the mean CT values obtained from qPCR assays performed using plasmid DNAs...

Results from quantitative PCR (qPCR) on fecal samples and cloacal and fecal float results from experimentally infected and wild caught northern bobwhites (Colinus virginianus).

Whitney M. Kistler (3235047)
Julie A. Parlos (3366101)
Steven T. Peper (633528)
Nicholas R. Dunham (633527)
Ronald J. Kendall (633530)

November 2016

Results from quantitative PCR (qPCR) on fecal samples and cloacal and fecal float results from ex...

Multiplex qPCR Data.

Heather L. Farrington (695021)
Christine E. Edwards (695022)
Xin Guan (6987)
Matthew R. Carr (695023)
Kelly Baerwaldt (695024)
Richard F. Lance (695025)

February 2015

Results of multiplexing trials of qPCR markers. Average Ct values, with the range of copy numbers...

Fecal RT-qPCR results for candidate AIEC transcripts.

Yuanhao Zhang (577563)
Leahana Rowehl (577561)
Julia M. Krumsiek (763107)
Erika P. Orner (763108)
Nurmohammad Shaikh (763109)
Phillip I. Tarr (90158)
Erica Sodergren (48852)
George M. Weinstock (43281)
Edgar C. Boedeker (158288)
Xuejian Xiong (105663)
John Parkinson (4087)
Daniel N. Frank (158306)
Ellen Li (158263)
Grace Gathungu (763110)

June 2015

The number of positive fecal stool samples are shown for each candidate AIEC transcript. Transcri...

Correlation between faecal egg counts and real-time PCR egg estimates.

Nichola Eliza Davies Calvani (4439005)
Peter Andrew Windsor (3129513)
Russell David Bush (4439008)
Jan Šlapeta (77953)

September 2017

(A) Real-time PCR egg estimates (qEPG) for three methods of sample preparation for DNA isolation ...

RT-qPCR results following the extraction-based and homogenization-based protocols represented as Ct values for each patient sample.

Zachary P. Morehouse (3832333)
Lyson Samikwa (7553132)
Caleb M. Proctor (11292285)
Harry Meleke (11292288)
Mercy Kamdolozi (3172389)
Gabriella L. Ryan (11292291)
David Chaima (3515378)
Antonia Ho (10214054)
Rodney J. Nash (11292294)
Tonney S. Nyirenda (11292297)

August 2021

RT-qPCR results following the extraction-based and homogenization-based protocols represented as Ct ...

Comparison of the egg recovery rates and limit of detection for soil-transmitted helminths using the Kato-Katz thick smear, faecal flotation and quantitative real-time PCR in human stool.

Patsy A Zendejas-Heredia
Vito Colella
Sze Fui Hii
Rebecca J Traub

May 2021

BackgroundMonitoring the success of soil-transmitted helminth (STH) control programs relies on accur...

Comparison of the egg recovery rates and limit of detection for soil-transmitted helminths using the Kato-Katz thick smear, faecal flotation and quantitative real-time PCR in human stool

Zendejas-Heredia, PA
Colella, V
Hii, SF
Traub, RJ

May 2021

BACKGROUND: Monitoring the success of soil-transmitted helminth (STH) control programs relies on acc...

Blood collection tube study results comparing qPCR to ddPCR.

Rebecca C. Holmberg (442521)
Alissa Gindlesperger (442522)
Tinsley Stokes (442523)
David Lopez (442524)
Lynn Hyman (442525)
Michelle Freed (442526)
Phil Belgrader (442527)
Jeanne Harvey (442528)
Zheng Li (26302)

August 2013

Correlation plot of percent fetal, % Y (ddPCR vs. qPCR) with a linear fit.</p

Results of qPCR for fecal card samples vs. stool/dilution samples for different egg counts.

Abstract

Extracted data

Results of qPCR for fecal card samples vs. stool/dilution samples for different egg counts.

Abstract

Extracted data

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