The levels of 4 hydroxynonenal (4-HNE) conjugated proteins in the diaphragm at 12 hours were measured via western blotting as an index of lipid peroxidation.

<p>A representative blot for 4-HNE protein conjugates is shown below the graph. The multiple bands represent different proteins modified by HNE-adducts, and give a general index of oxidative damage. Values are means ± SE and normalized to percent control. Symbols: * significantly different (p<0.05) from all other groups.