Dimeric pAbs bind more tightly to PFs than their monomeric form.

<p>(<b>A</b>) Left panel: SEC chromatograms for ~1 mg/mL of protein A-purified pAbs and IVIg in PBS, pH 7.4. SEC was carried out using a Hiprep16/60 Sephacryl S300 HR column (GE Healthcare) that was equilibrated in PBS, pH 7.4. Arrows indicate the elution of protein molecular weight standards. Right panel: Antibody binding curves against PFs for unfractionated (Unfrac) and SEC-isolated monomeric pAbs. (<b>B</b>) Left panel: Antibody binding curves against PFs for unfractionated and SEC-isolated monomeric and dimeric IVIg IgGs. Right panel: SEC chromatographs for ~0.2 mg/ml IVIg dimers and monomers in PBS, pH 7.4, after incubation under ELISA-like conditions (4 h at 37°C). (<b>C</b>) Dynamic light scattering for 0.8 mg/mL and 0.06 mg/mL of SEC-isolated IVIg monomers and (<b>D</b>) dimers, respectively. Dynamic light scattering was determined at room temperature immediately after SEC-isolation of each IgG conformer in PBS, pH 7.4. <i>R</i>_<i>h</i> and pd are abbreviations for hydrodynamic radius and polydispersity, respectively.