The rate of photooxidation/photodegradation of A2E varies with the composition of phospholipid liposomes.

<p>(A) A2E (50 μM) was incorporated into phosphatidylcholine vesicles having the following fatty acid chains: DPPC (1,2-dipalmitoyl-<i>sn</i>-glycero-3-phosphatidylcholine); DOPC (1, 2- dioleoyl- <i>sn</i>- glycero- 3- phosphatidylcholine); PO-PC (1- palmitoyl- 2- oleoyl- <i>sn</i>- glycero- 3- phosphatidylcholine) and to mixed vesicles of DOPC, DOPS (1, 2- dioleoyl- <i>sn</i>- glycero- 3- phosphatidylcholine); and DOPE (1, 2- dioleoyl- <i>sn</i>- glycero- 3- phosphatidylethanolamine) (ratio of 65:25:10). Vesicles were prepared in phosphate buffered saline and irradiated for 1, 5, 10 and 20 mins and remaining A2E was analyzed by HPLC with monitoring at 430 nm absorbance. Comparison was made to A2E in PBS with 1% DMSO. (B) Rate constants for A2E photodegradation in the presence of the indicated phospholipid liposomes. Means ± (SEM) of 3 replicates. Error bars that are not visible do not extend outside the symbol.