GPMVs from RBL-2H3 exhibit a broad distribution of miscibility transition temperatures.

<p>(A) A single field of GPMVs imaged at several distinct temperatures. Vesicles identified as containing coexisting liquid phases are marked with a yellow triangle. In this field, some vesicles contain coexisting phases and others are uniform at both 20° and 24°C. (B) Heterogeneity in the transition is quantified in a single GPMV preparation by measuring the fraction of vesicles containing two coexisting liquid phases as a function of temperature over many fields of vesicles like those shown in A. The average transition temperature is defined as the extrapolated temperature where 50% of vesicles are phase separated, which in this case is 21.0±0.2°C (dashed line). The width of the transition typically spans 10°C.