Transition temperatures vary systematically as RBL-2H3 cells synchronized to the G1/S boundary progress through the cell cycle.

<p>(A) Representative fields of GPMVs prepared at the indicated time-points after release from a double thymidine block and imaged at 23°C. GPMVs isolated immediately after release from block (top) are mostly in a single phase whereas GPMVs isolated at later time-points often contain two phases in coexistence. (B) The fraction of phase separated GPMVs as a function of temperature for GPMVs isolated at the indicated times after release from block. These curves are used to quantify the average transition temperature (T_misc). The dashed line is drawn at 23°C for comparison to A. (C) T_misc as a function of time after release from the double thymidine block. Larger colored symbols represent values obtained in B and black symbols include data from 4 separate trials. The black line is smoothed from black points using a lowess filter.