T_misc is reduced in GPMVs from apoptotic cells.

<p>(A) GPMVs isolated from RBL-2H3 cells pretreated with 100ng/ml TRAIL for 30min have lower transition temperatures than GPMVs isolated from untreated cells. At elevated temperatures, some TRAIL treated GPMVs contain non-circular domains. (B) GPMVs isolated from RBL-2H3 cells pretreated with purified sphingomyelinase (SMase) contain rigid and elongated gel domains at elevated temperature and more rigid liquid-like domains at lower temperature. GPMVs from sphingomyelinase treated cells have lower T_misc than control GPMVs, where T_misc is defined as the onset of the liquid appearing domains. (C) Elongated gel domains are also observed in GUVs containing purified DOPC, BSM, and Chol when 4–5 mol% of BSM is replaced by Brain ceramide. Images were acquired at 25°C.