RhoGAP18B isoforms bind different members of the Rho-family of GTPases.

<p>Co-immunoprecipitation blots showing pull-down of GFP-tagged Rho-type GTPases with FLAG-tagged PC, FLAG-tagged PD, or HA-tagged PA. Rho-type GTPases pulled down with anti- FLAG or anti-HA beads was then detected with anti-GFP antibody. All isoforms preferentially bind to the constitutive active forms of Rho-family GTPases. PA binds to Cdc42 only; PD binds Rac1 and, to a lesser extent, Rho; and PC binds to all three GTPases. Representative blots from one of 3–5 independent experiments are shown.