Two h after the end of resuscitation, liver tissue was harvested and western blot for the phosphorylated or non- phosphorylated c-JUN (Fig 6A), p65 subunit of NF-κB and β-actin was performed.

<p>Lanes 1–4: liver protein extracts from ctrl-fed mice treated with veh, lanes 5–8: ctrl-fed mice treated with D-JNKI-1, lanes 9–12: EtOH-fed mice treated with veh and lanes 13–16: EtOH-fed mice treated with D-JNKI-1. Sham operated animals underwent the surgical procedures but hemorrhagic shock with resuscitation (H/R) was not carried out. In Fig 6B, the ratio of phosphorylated c-JUN and p65 subunit of c-JUN and NF-κB, respectively, and total protein after densitometric measurements and normalization to β-actin is represented. (*: p <0.05 <i>vs</i>. corresponding sham group, #: p <0.05 <i>vs</i>. corresponding H/R group).