Leaf anatomical study and microscopic view of epidermal impression of transgenic and wild type plants (40X magnification).

<p>A) Leaf cross-sections observed under microscope. For histological studies, the top third leaf of transgenic and wild type plants was taken and microtome sections were made. Staining was done using eosin (stains nucleus) and hematoxylin (stains cytoplasm). B) Photograph showing abaxial surface of leaves from transgenic and wild type plants. C) Graph depicting stomatal index of transgenic and wild type plants. Data represent mean of three replications (n = 3) and bars indicate standard error of mean. The lowercase letters that are different indicate significant difference (Duncan’s multiple range test, P<0.05) between transgenic and wild type plants. x- xylem, p-phloem, S-spongy parenchyma, P-palisade parenchyma, ad-Adaxial, ab- abaxial.