DNA response induced by 5-Fu, Gyp and 5-Fu + Gyp.

<p>(A) DNA fragmentation was detected using flow cytometry after 5-Fu, Gyp and 5-Fu + Gyp treatment in SW-480 cells after 24 and 48 h. Histograms showed number of cell channels (vertical axis) vs. PI fluorescence (horizontal axis). (B) Comet assay was performed for detection of DNA damage after 5-Fu co-treated with Gyp. (C) The expression level of Ser139-Histone H2A.X was determined using western blotting. Experiments were done independently in triplicate per experimental point, and representative results were shown. All data are expressed as means ± SD of triplicates and <i>p</i>* < 0.05, <i>p</i>** < 0.01 versus control; <i>p</i>^<i>##</i> < 0.01 versus 5-Fu or Gyp alone group.