Augmented IL-1β expression during viral FH.

<p><b>(A)</b> Cultured peritoneal exudative macrophages (PEMs) and RAW264.7 cells were infected with MHV-3 (MOI = 1) <i>in vitro</i>, and IL-1β p17 protein levels were detected in the indicated time points by western-blotting. C57BL/6 WT mice were infected with MHV-3 (100PFU), <b>(B)</b> PEMs and livers were isolated and the transcription of <i>proIL-1β</i> mRNA was measured by qPCR. *<i>p</i><0.05, NS: no significant difference. <b>(C)</b> The expression of proIL-1β and IL-1β p17 in virus-infected liver tissues was detected by western-blotting. Three representative samples <i>per</i> group are shown. <b>(D)</b> The proIL-1β protein in PEMs isolated from MHV-3 infected mice at the indicated time points was detected by flow cytometry. The up panels are gate strategies and number indicates the percentage of positive cells in the gate. One representative sample from five mice <i>per</i> group is showed. <b>(E)</b> Serum IL-1β and <b>(F)</b> IL-1α levels in virus-infected mice were detected by ELISA. N = 5~10 <i>per</i> group,*<i>p</i><0.05, NS: no significant difference.