Cytokine expression kinetics and the effect of different Golgi inhibition periods.

<p>Three alternative Golgi inhibition periods (A, B and C) with different times of commencement and duration will detect different cytokine secretion profiles when cytokine responses of differing secretion kinetics (green, red, yellow and blue lines) are to be measured. Longer Golgi inhibition periods that start earlier in the stimulation period (i.e. B), are most likely to allow the detection of polyfunctional profiles. A and C are more likely to miss cytokines with early, short expression periods (e.g. green) but later inhibition periods such as C are more likely to detect cytokines with late expression kinetics (e.g. blue).