Effect of PTU on serum-induced PASMC proliferation and migration.

<p>After 24 hours of serum deprivation, PASMCs from MCT rats were treated with indicated conditions for 24 hours. Translocation of proliferating cell nuclear antigen (PCNA, red staining; scale bar: 75μm) into the nucleus <b>(A)</b> and BrdU incorporation <b>(B)</b> were used to represent proliferative activities of PASMCs. Each value (mean±SE [n = 6]) is expressed as fold change of BrdU incorporation in control cells with 0.1% FBS. (<b>C)</b> Migratory activity of PASMC was assessed by migration assay chamber. After treatment with indicated conditions for 6 hours, PASMCs from MCT rats at the lower aspect of filter membrane was fixed and stained with Liu’s stain (pink staining; magnification 100X). (<b>D)</b> Each value (mean±SE [n = 6]) is determined by cell number at the lower aspect of filter membrane, and expressed as a percentage of control. *p<0.05, **p<0.01, ***p<0.001 versus 10% FBS without PTU, one-way ANOVA, bonferroni’s post test.