Effects of post translational modifications on the periplasmic loop of MscL determined by <i>in vivo</i> channel activity.

<p>The viability of the osmotically fragile strain MJF455 expressing MscL cysteine substituted mutants from residues L47 to H74 (insert), was measured after an osmotic down-shock. The graphs show the differences in viability between non-treated and post-transnationally modified channels with (A) the hydrophobic MTS reagents MTSBn (blue), decyl-MTS (red) or 4HB-MTS (green) or (B) the negatively charged MTSES^- (blue) and positively charged MTSET⁺ (red). The red grid line indicates a ± 50% change that was used as a threshold for further studies.