<p>MH-S cells were infected with PBS or SP-D treated <i>C</i>. <i>burnetii</i> RSA439 (phase II), RSA493 (phase I) or treated with <i>E</i>. <i>coli</i> LPS for 0, 4, and 8 hours. RNA was extracted as described and applied to real-time PCR. Data represent the mean ± standard deviation of fold change in RNA expression of select genes compared to expression of host cell <i>Gapdh</i>, n = 3. RSA439 and LPS data are representative of three independent experiments, RSA493 data are representative of two independent experiments. Student t-tests compare each time and condition to its corresponding 0 time point, all RSA439 and RSA439 + SP-D were significantly up-regulated * p<0.05. There was no significant difference in expression between untreated ...
<p>rhSP-D treatments resulted in an increase of SP-A1, SP-A2 and SP-D mRNA expression at 6 h at 5 an...
<p><i>A</i> to <i>C</i>, Peritoneal macrophages were isolated 2 hours post injection from LPS-challe...
<p><b>(A, B)</b> Heatmaps of the significantly regulated blood and spleen transcripts at day 3 follo...
<p>(A) <i>E</i>. <i>coli</i> were treated with or without SP-D for one hour at 37°C followed by viab...
<p>Transcript level responses measured by quantitative RT-PCR analysis 24 hrs after LPS treatment of...
<p>Wild-type <i>C</i>. <i>burnetii</i> growth in infected MH-S cells treated with different inhibito...
<p><b>(A)</b> Immunoblot of HEK 293 cells with knockdown of VPS35 by siRNA. A non-targeting pool (NT...
<p>Transcript level responses measured by quantitative RT-PCR analysis 24 hrs after exposure to 2 Gy...
<p>(A) HeLa cells were cotransfected with pEGFP-mDia1 WT (panels a-j) or pEGFP-mDia1-ΔN3 (constituti...
<p>(<b>A</b>) Quantitative comparison of (>2-fold) up- and downregulated gene expression changes in ...
<p>(A, B, D) Microarray data show LPS-induced time-dependent changes in the indicated transcripts. F...
<p>mRNA (A) was analyzed by quantitative Real-Time PCR. Normalized gene expression levels were given...
<p>(A) HeLa cells were co-transfected with pEGFP and a scramble (panels a and b), Rac1 (panels c and...
<p>THP-1 cells were infected with NMII at MOI 5 or 50 and left untreated or treated with staurospori...
<p>Quantitative real-time PCR analysis was carried out on RWPE-1, PC3, DU145 and LNCaP C-81 cells tr...
<p>rhSP-D treatments resulted in an increase of SP-A1, SP-A2 and SP-D mRNA expression at 6 h at 5 an...
<p><i>A</i> to <i>C</i>, Peritoneal macrophages were isolated 2 hours post injection from LPS-challe...
<p><b>(A, B)</b> Heatmaps of the significantly regulated blood and spleen transcripts at day 3 follo...
<p>(A) <i>E</i>. <i>coli</i> were treated with or without SP-D for one hour at 37°C followed by viab...
<p>Transcript level responses measured by quantitative RT-PCR analysis 24 hrs after LPS treatment of...
<p>Wild-type <i>C</i>. <i>burnetii</i> growth in infected MH-S cells treated with different inhibito...
<p><b>(A)</b> Immunoblot of HEK 293 cells with knockdown of VPS35 by siRNA. A non-targeting pool (NT...
<p>Transcript level responses measured by quantitative RT-PCR analysis 24 hrs after exposure to 2 Gy...
<p>(A) HeLa cells were cotransfected with pEGFP-mDia1 WT (panels a-j) or pEGFP-mDia1-ΔN3 (constituti...
<p>(<b>A</b>) Quantitative comparison of (>2-fold) up- and downregulated gene expression changes in ...
<p>(A, B, D) Microarray data show LPS-induced time-dependent changes in the indicated transcripts. F...
<p>mRNA (A) was analyzed by quantitative Real-Time PCR. Normalized gene expression levels were given...
<p>(A) HeLa cells were co-transfected with pEGFP and a scramble (panels a and b), Rac1 (panels c and...
<p>THP-1 cells were infected with NMII at MOI 5 or 50 and left untreated or treated with staurospori...
<p>Quantitative real-time PCR analysis was carried out on RWPE-1, PC3, DU145 and LNCaP C-81 cells tr...
<p>rhSP-D treatments resulted in an increase of SP-A1, SP-A2 and SP-D mRNA expression at 6 h at 5 an...
<p><i>A</i> to <i>C</i>, Peritoneal macrophages were isolated 2 hours post injection from LPS-challe...
<p><b>(A, B)</b> Heatmaps of the significantly regulated blood and spleen transcripts at day 3 follo...