MyD88 signaling potentiates proliferation of Tc17 cells.

<p>Naïve WT or MyD88ΔT mice were CD4⁺ T cell depleted and vaccinated with strain #55. BrdU was pulsed through the drinking water on indicated interval days. Mice were sacrificed and dLNs were harvested at the end of each indicated pulse period. Cells were restimulated, stained for surface and intracellular cytokines before BrdU staining. Percent BrdU^+ve cells were analyzed by flow cytometry by gating on CD8⁺ IL-17- or IFNγ-producing cells. CD4⁺ T cells were depleted throughout the experiment. Values are percent mean ± SD of 4–5 mice/group. *P≤0.05. Data is representative of 3 independent experiments.