Rapamycin treatment blunts Tc17 cell responses and proliferation.

<p>Naïve WT and MyD88ΔT mice were CD4⁺ T cell depleted and vaccinated with strain #55. Rapamycin (~2 μg/mouse) was administered daily from day 4 by the i.p. route. On day 19 post-vaccination, dLNs and spleens were harvested to enumerate percent and total numbers of IL-17 and IFNγ producing CD8⁺ T cells <b>(A)</b>. Cohorts of mice were pulsed with BrdU through drinking water from day 7–15. On day 16, dLNs and spleens were harvested to enumerate percent BrdU^+ve IL-17A and IFNγ producing CD8⁺ T cells <b>(B)</b>. Values are the mean ± SD of 4–7 mice/group. *p≤0.05, ***p≤0.001 and ****p≤0.0001. Data is representative of 2 independent experiments.