<p>a. Level of mRNA normalized with an internal control (<i>GAPDH</i>).</p><p>b. Cells transfected with a mix of 3 scrambled siRNAs. The optimal concentration of siRNAs that greatest decreases the <i>CDKN3</i> messenger was 80 nM for SiHa and CaSki, and 100 nM for HeLa cell lines.</p><p>c. Cells transfected with a mix of 3 specific siRNAs against <i>CDKN3</i>. The optimal concentration of siRNAs that greatest decreases the CDKN3 messenger was 80 nM for SiHa and CaSki, and 100nM for HeLa cell lines.</p><p>d. % of Decrease = 100—(siRNA <i>CDKN3</i>/siRNA Control)*100</p><p>e. t test.</p><p><i>CDKN3</i> gene expression decreased in cervical cancer cell lines transfected with specific siRNAs against <i>CDKN3</i>.</p
<p>HeLa cells were transfected with ten siRNA targeting CSNK2A, and two control siRNA, (GFP as a neg...
We describe the protocols of using siRNAs, or shRNAs delivered by a lentiviral vector, as a means to...
<p>A) Vero E6 cells were transfected with Chik-1 and Chik-5 siRNAs individually as well as in combin...
<p>Cell lines derived from cervical cancer (CC) positive for human papilloma virus (HPV) 16 (CaSki, ...
<p>a. Calculated using the equations of exponential growth, included in <a href="http://www.plosone....
<div><p>The cyclin-dependent kinase inhibitor 3 (<i>CDKN3</i>) gene, involved in mitosis, is upregul...
The cyclin-dependent kinase inhibitor 3 (CDKN3) gene, involved in mitosis, is upregulated in cervica...
<p>The expression of cyclin-dependent kinase inhibitor 3 (<i>CDKN3</i>) was measured by RT-qPCR in 2...
<p>(A) Specific STAT3 siRNA inhibits STAT3 and pSTAT3 expression in cervical cancer cells. Cellular ...
<p>Cell lines were transfected with specific <i>CDKN3</i> (black bars) or scrambled siRNAs (white ba...
<p>(a) Real-time polymerase chain reaction and western blot analysis of cancer cells transfected wit...
<p>293T cells were transfected with 50 µg of plasmid expressing the HCMV siRNA target transcript 24 ...
<p>(A) All siRNA transfections were performed or not (control, C) with 12.5 nM siRNAs directed again...
<p>(A) HeLa cells were transfected with siRNA combinations (11.7pmol of each siRNA) against HDAC3/TB...
Human papillomavirus (HPV) is the infectious agent responsible for the development of cervical cance...
<p>HeLa cells were transfected with ten siRNA targeting CSNK2A, and two control siRNA, (GFP as a neg...
We describe the protocols of using siRNAs, or shRNAs delivered by a lentiviral vector, as a means to...
<p>A) Vero E6 cells were transfected with Chik-1 and Chik-5 siRNAs individually as well as in combin...
<p>Cell lines derived from cervical cancer (CC) positive for human papilloma virus (HPV) 16 (CaSki, ...
<p>a. Calculated using the equations of exponential growth, included in <a href="http://www.plosone....
<div><p>The cyclin-dependent kinase inhibitor 3 (<i>CDKN3</i>) gene, involved in mitosis, is upregul...
The cyclin-dependent kinase inhibitor 3 (CDKN3) gene, involved in mitosis, is upregulated in cervica...
<p>The expression of cyclin-dependent kinase inhibitor 3 (<i>CDKN3</i>) was measured by RT-qPCR in 2...
<p>(A) Specific STAT3 siRNA inhibits STAT3 and pSTAT3 expression in cervical cancer cells. Cellular ...
<p>Cell lines were transfected with specific <i>CDKN3</i> (black bars) or scrambled siRNAs (white ba...
<p>(a) Real-time polymerase chain reaction and western blot analysis of cancer cells transfected wit...
<p>293T cells were transfected with 50 µg of plasmid expressing the HCMV siRNA target transcript 24 ...
<p>(A) All siRNA transfections were performed or not (control, C) with 12.5 nM siRNAs directed again...
<p>(A) HeLa cells were transfected with siRNA combinations (11.7pmol of each siRNA) against HDAC3/TB...
Human papillomavirus (HPV) is the infectious agent responsible for the development of cervical cance...
<p>HeLa cells were transfected with ten siRNA targeting CSNK2A, and two control siRNA, (GFP as a neg...
We describe the protocols of using siRNAs, or shRNAs delivered by a lentiviral vector, as a means to...
<p>A) Vero E6 cells were transfected with Chik-1 and Chik-5 siRNAs individually as well as in combin...