Apoptosis analysis of AML cell lines.

<p>The indicated cell lines were incubated with (A) Nutlin-3a (0, 0.5, 1.0, 2.5, 5, or 10 μM), Ara-C (0, 0.5, 1.0, 2.5, 5, 10, or 20 μM), KPT-330 (0, 50, 100, 250, 500, 1000 nM in U937, MOLM-13, MOLM-13 and MV4;11 cells. 0, 50, 100, 250, 500, 1000, 2500, 5000, and 10,000 nM in HL-60, KG-1, MOLM-16, THP-1, U-937 and OCI-AML3 cells), or (B) ABT-199 (0, 50, 100, 250, 500, 1000 nM in MOLM-13 and MV4;11 cells. 0, 50, 100, 250, 500, 1000, 2500, 5000, and 10,000 nM in HL-60, KG-1, MOLM-16, THP-1 and OCI-AML3 cells) for 48 hours. The annexin V-positive cell fractions and annexin V- and propidium iodide-negative live cells were counted using flow cytometry. The y axes of left graphs show the extent of drug-specific apoptosis, calculated as described in the method section. The standard deviations were confirmed to be less than 5% of each mean value by conducting three independent experiments in triplicate to assess the Annexin V-positive and live cell numbers.