Migratory activity of hCPCs kept as monolayers or of cells migrated from seeded spheroids (wound healing assay).

<p>(<b>A</b>) Quiescent cell monolayers were “wounded” with a pipette tip and incubated for 24 hours in the absence (C-) or presence of 20% FBS (C+) or of different concentrations of the HGF receptor agonist mAb DO-24 (5, 20, 80 nM respectively). (<b>B</b>) Quantitative analysis calculated as % variation of cell numbers in the rectangles depicted in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0137999#pone.0137999.g005" target="_blank">Fig 5A</a> relative to untreated samples. The assay was repeated three times with triplicates and one representative assay is reported. Images were taken at 5x magnification. After 24 hours healing was significantly induced by 20% FBS and mAb DO-24 treatments. Cells derived from spheroids (dark grey bars) were more efficient than cells from 2D cultures at any condition (light grey bars).