<p>Different concentrations of Ts2631 endolysin were added to chloroform treated <i>T</i>. <i>thermophilus</i> HB8 DSM 579 cell suspension in 200 μl of 10 mM potassium phosphate buffer pH 8.0 and a decrease in OD<sub>600</sub> was measured for 40 min in 5 min intervals. Results of the control (buffer only) were subtracted from the sample values. The experiment was repeated in triplicate; error bars indicate the standard deviation.</p
<p>The mean log<sub>10</sub>colony-forming unit (CFU) reduction of <i>C</i>. <i>difficile</i> spores...
<p>A) Lysate from WT cells were deposited on an agarose plate containing cell wall extracts of <i>M....
<p>U937 and K562 cells were treated with 50 mM ammonium chloride (A), 2.5 mM methyl-β-cyclodextrin (...
<p>Relative activity against <i>T</i>. <i>thermophilus</i> HB8 cells was calculated by comparing the...
<p>Relative activities are expressed as the percentage of activity to <i>T</i>. <i>thermophilus</i> ...
<p>PlyPl23 activity on <i>P</i>. <i>larvae</i> Pl02-23 at different pH values (pH 5.0, 6.0, and 7.0)...
<p>PlyPl23 activity on <i>P</i>. <i>larvae</i> Pl02-23 at different pH values, ranging from 5 to 9. ...
<p>(A) Clear lysates of Ts2631 endolysin substitution variants (1) and supernatants after heat treat...
<p>A) To measure the cell lysis activities of P5<sup>wt</sup> and P5<sup>V207F</sup> the turbidity o...
<p><b>A)</b> Lysis of a 1 mg/ml <i>Micrococcus lysodeikticus</i> cell suspension (expressed as a red...
<p>DCs that were induced using different culture methods were co-cultured with FITC-dextran 40,000 f...
<p>The protein suspension extract (about 13 mg) from the phage lysate was mixed with sample buffer w...
<p>Tac-furin cells were pretreated with 100 µM Leupeptin (Leu), E-64, Chymostatin (Chy), a combinati...
<p>The bar graphs show the effects of different inhibitors and temperature on cellular uptake of (a)...
<p>Dextran-FITC uptake at 37°C was evaluated by flow cytometry. DC were incubated for 48 h in ECM-co...
<p>The mean log<sub>10</sub>colony-forming unit (CFU) reduction of <i>C</i>. <i>difficile</i> spores...
<p>A) Lysate from WT cells were deposited on an agarose plate containing cell wall extracts of <i>M....
<p>U937 and K562 cells were treated with 50 mM ammonium chloride (A), 2.5 mM methyl-β-cyclodextrin (...
<p>Relative activity against <i>T</i>. <i>thermophilus</i> HB8 cells was calculated by comparing the...
<p>Relative activities are expressed as the percentage of activity to <i>T</i>. <i>thermophilus</i> ...
<p>PlyPl23 activity on <i>P</i>. <i>larvae</i> Pl02-23 at different pH values (pH 5.0, 6.0, and 7.0)...
<p>PlyPl23 activity on <i>P</i>. <i>larvae</i> Pl02-23 at different pH values, ranging from 5 to 9. ...
<p>(A) Clear lysates of Ts2631 endolysin substitution variants (1) and supernatants after heat treat...
<p>A) To measure the cell lysis activities of P5<sup>wt</sup> and P5<sup>V207F</sup> the turbidity o...
<p><b>A)</b> Lysis of a 1 mg/ml <i>Micrococcus lysodeikticus</i> cell suspension (expressed as a red...
<p>DCs that were induced using different culture methods were co-cultured with FITC-dextran 40,000 f...
<p>The protein suspension extract (about 13 mg) from the phage lysate was mixed with sample buffer w...
<p>Tac-furin cells were pretreated with 100 µM Leupeptin (Leu), E-64, Chymostatin (Chy), a combinati...
<p>The bar graphs show the effects of different inhibitors and temperature on cellular uptake of (a)...
<p>Dextran-FITC uptake at 37°C was evaluated by flow cytometry. DC were incubated for 48 h in ECM-co...
<p>The mean log<sub>10</sub>colony-forming unit (CFU) reduction of <i>C</i>. <i>difficile</i> spores...
<p>A) Lysate from WT cells were deposited on an agarose plate containing cell wall extracts of <i>M....
<p>U937 and K562 cells were treated with 50 mM ammonium chloride (A), 2.5 mM methyl-β-cyclodextrin (...