Comparison of the effect of anticancer drugs on short- and long-term proliferation of breast cancer cell lines.

<p>(<b>A</b>) Morphology of control and treated breast cancer cells indicative of growth arrest phenotype. (<b>B</b>) Colony formation assay of control and treated breast cancer cells (MCF7) showing decrease in treated cells. (<b>C</b>) Quantitation of the colony-forming assay from three independent experiments. (<b>D</b>) Comparative effect of embelin on cancer (MCF7) and normal (TIG-3) cells as detected by short term (viability) and long term (colony forming efficiency) growth assays. Statistical significance was calculated by QuickCals t-test calculator (***P<0.001).