Effect of TNF-α inhibition with etanercept on Ang II-induced AT₁R gene expression, NF-κB activity, and phosphorylation of p38 MAPK and JNK in WT mice.

<p>(a) AT₁R gene expression was significantly elevated in heart tissue by Ang II infusion, which was attenuated by etanercept treatment. (b) Chronic Ang II infusion significantly increased cardiac NF-κB p65 activity in WT mice but not in mice treated with etanercept. (c) p38 MAPK and (d) JNK phosphorylation in the hearts of Ang II-infused mice was significantly increased. Etanercept treatment prevented this Ang II-induced phosphorylation. Values are mean ± SEM. *<i>p</i>< 0.05 vs WT+Saline, # <i>p</i>< 0.05 vs WT+Ang II, n = 6 per group.