DR5 and CASP8 expression are top ranking features correlated with sensitivity and together improve predictions.

<p>(<i>A</i>) Differential association of gene expression using p-values to assess the significance of gene expression features correlated with DR5Nb1-tetra sensitivity. Inset shows the top 20 genes (dotted line = FDR < 0.05). Colors are based on association with sensitives (red) and insensitives (blue). (<i>B</i>) Comparison of relative surface protein levels of DR5 to mRNA expression in 25 pancreatic cancer cell lines. Points are colored based on sensitivity to DR5Nb1-tetra: sensitives (red), intermediates (orange) and insensitives (blue). <i>(C)</i> Induction of Casp8 activity compared to DR5 gene expression in 27 pancreatic cell lines. (<i>D</i>) DR5+Casp8 expression compared to sensitivity. DR5 and Casp8 are individually significantly associated with response, as shown in the marginal histograms for sensitives (red), insensitives (blue), with overlap shown in purple (DR5: p = 10⁻¹², Casp8: p = 10⁻⁹). Scatterplot of DR5 and Casp8 shows that cell lines with high expression of both genes (marked by red box) are enriched in sensitives (PPV = 44%) compared to high DR5 (PPV = 34%) and unselected (PPV = 34%).