The effect of the nucleotide at position -3 on gene expression.

<p>(A) The bases at the 5’-end of transcripts were shown schematically and the mutational bases were in grey. The <i>β</i>-galactosidase specific activities (BgaH activities), the <i>bgaH</i> transcript levels and the translational efficiencies of <i>Hfx</i>. <i>volcanii</i> transformants were tabulated. (B) The translational efficiencies of (A) were shown schematically after normalization to that of <i>Hfx</i>. <i>volcanii</i> DS70/pTMJ. (C) Colonies of <i>Hfx</i>. <i>volcanii</i> transformants sprayed with X-Gal. The strains were cultivated for 5 days and then sprayed with X-Gal. The constructs present in each transformant were indicated under the colonies.