Autophagosome Proteins LC3A, LC3B and LC3C Have Distinct Subcellular Distribution Kinetics and Expression in Cancer Cell Lines - Fig 4

<p><b>(A)</b> Cytoplasmic and nuclear staining of LC3C (red) in U87 cell line. <b>(B)</b> Double LC3C (red) and LC3B (green) immunostaining in U87 cells showing lack of co-localization between the two proteins, both in the cytoplasm and the nuclear/nucleolar regions. <b>(C,D)</b> Double LC3C (red) and LC3A (green) immunostaining showing co-localization between the two proteins, mainly in the nuclear area (U87 and T98 cell lines). <b>(E)</b> Western blot for LC3C in the nuclear (N), Pellet (P) and Soluble (S) fraction of U87 and T98 cells. ‘M’ is the marker and Lamin is used as a control to confirm the presence of nuclei only in the nuclear fraction.