Agglutination assay of GM1-Glc-immobilized fluorescent nanoparticles (GM1-Glc-SFNPs) with sera from GBS patients.

<p>(A) Visual image of fluorescent aggregates under UV irradiation (samples 1 and 3: negative for anti-GM1/GD1a antibodies; sample 2: positive for anti-GD1a antibodies; samples 4, 5, and 6: positive for anti-GM1 IgG antibodies). (B) Fluorescence spectra of supernatants monitored by excitation at 360 nm. (C) Silver-stained SDS-PAGE of aggregates obtained from sample 6; 10% gel with 2-mercaptoethanol (2-ME; left) and 8% gel without reducing reagents (right). Lanes 1 and 4: GM1-Glc-SFNP; lanes 2 and 5: aggregates of GM1-Glc-SFNPs; lanes 3 and 6: diluted serum. (D) Western blotting analysis of fluorescent aggregates of GM1-Glc-SFNPs; 10% gel with 2-mercaptoethanol. Lane 1: aggregates of GM1-Glc-SFNPs; lanes 2: diluted serum.