Human neutrophils migrate to fMet-Leu-Phe and PT-gliadin.

<p>(A-C) Data were obtained using the EZ-TAXIScan assay. (A) Images from representative EZ-TAXIScan movies (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138338#pone.0138338.s004" target="_blank">S3 movie</a>). (B) Image shows the paths of individual cell migration to PT-water, PT-zein, PT-gliadin or fMet-Leu-Phe. The colors progress from red to blue as a function of time. (C) Quantitative analysis of all EZ-TAXIScan assay results of human neutrophil chemotaxis in response to PT-water, PT-zein, PT-gliadin and fMet-Leu-Phe. The table depicts speed (μm/min) and the chemotactic index (CI). PT-gliadin induced neutrophil chemotaxis with speed and CI similar to fMet-Leu-Phe (<i>P</i> = NS). Data are representative of results obtained from four independent movies. (D) PT-gliadin induced neutrophil migration in a dose-dependent manner in a Transwell chemotaxis assay (<i>P</i><0.01). Data are obtained from six independent experiments. (E) PT-gliadin induced neutrophil migration in a dose-dependent manner in the EZ-TAXIScan assay. As a positive control, fMet-Leu-Phe was used. Data are obtained from three independent experiments.