Phosphorylation levels of ERK1/2 and AKT in SEMA7A knockdown cells with/without TGF-β<sub>1</sub>.
- Tomoaki Saito (797073)
- Atsushi Kasamatsu (281849)
- Katsunori Ogawara (281852)
- Isao Miyamoto (797074)
- Kengo Saito (593605)
- Manabu Iyoda (359431)
- Takane Suzuki (797075)
- Yosuke Endo-Sakamoto (359437)
- Masashi Shiiba (281853)
- Hideki Tanzawa (145219)
- Katsuhiro Uzawa (145207)
Publication date
September 2015
DOIAbstract
<p>Immunoblot analysis of the phosphorylation levels of SEMA7A, ERK1/2, and AKT. SEMA7A knockdown results in decreased levels of pERK1/2 and pAKT compared with the shMock cells (SAS and KOSC-2-derived transfectants). TGF-β<sub>1</sub> treated shSEMA7A cells show increased SEMA7A level compared with TGF-β<sub>1</sub> non-treated shSEMA7A cells. The pERK1/2 and pAKT level also show increased in TGF-β<sub>1</sub> treated shSEMA7A cells.</p
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