Additional file 16: Figure S9. of Phosphoproteome dynamics mediate revival of bacterial spores

Phospho-modifications of translation elongation factors affect vegetative growth. (A) Spores of PY79 (WT), AR165 (P hyper-spank -EF-G, ∆EF-G), AR166 (P hyper-spank -EF-G-Y339A, ∆EF-G), and AR167 (P hyper-spank -EF-G-Y339D, ∆EF-G) strains were incubated at 37 °C in S7-defined medium supplemented with L-Ala (10 mM) and 0.5 mM IPTG, and optical density (OD600) was measured at the indicated time points. Data are presented as a fraction of the initial OD600 of the phase-bright spores. Decreasing OD600 signifies spore germination while increasing OD600 indicates spore outgrowth. (B) Strains listed in (A) were grown at 37 °C in S7- supplemented with L-Ala (10 mM) and 0.5 mM IPTG, and OD600 was measured at the indicated time points. AR166 (P hyper-spank -EF-G-Y339A, ∆EF-G) and AR167 (P hyper-spank -EF-G-Y339D, ∆EF-G) strains showed significantly reduced growth rates compared to the control strains by repeated measures ANOVA (P <0.05). C) Spores of PY79 (WT), AR157 (P xyl -EF-TU, ∆EF-TU), AR158 (P xyl -EF-TU-Y270A, ∆EF-TU), and AR159 (P xyl -EF-TU-Y270D, ∆EF-TU) strains were incubated at 37 °C in S7- supplemented with L-Ala (10 mM) and 0.5 % xylose, and OD600 was measured at the indicated time points. Data are presented as a fraction of the initial OD600 of the phase-bright spores. (D) Strains listed in (C) were grown at 37 °C in S7- supplemented with L-Ala (10 mM) and 0.5 % xylose, and OD600 was measured at the indicated time points. The data points are averages of results obtained from three independent biological repeats. Error bars designate SD. AR159 (P xyl -EF-TU-Y270D, ∆EF-TU) showed significantly reduced growth rates compared to the other strains by repeated measures ANOVA (P