Effects of H-rev107 and POR siRNAs on triglyceride degradation.

<p>Huh7 cells were transfected with the indicated siRNA or not for 48 h, cell lysates were prepared, and the expression of H-rev107 (A) or POR (B) was determined using anti-H-rev107 or anti-POR antibodies. Cells were transfected with indicated siRNA (C) or treated with PLA₂ inhibitor (D) for 24 h and then incubated in 1% BSA/RPMI medium containing with oleic acid for 3 h. Cells were washed and incubated for another 24 h. The glycerol content in the media was determined by EIA. Representative results of three independent experiments are shown. *Indicates <i>p</i> value <0.05.