Intratumoral (i.t.) vaccination with recombinant 16E6E7 and 16E6E7m influenza viruses delays growth of, or eradicates established TC-1 tumors.

<p><b>A</b> Schematic illustration of the experimental set up <b>B</b> Five groups of female C57BL/6 mice (8 per group) were inoculated with 5x10⁴ TC-1 cells on day 0 and tumor growth monitored. After palpable tumors had developed, mice were primed i.t. on day 13 and 14 with PBS (mock), H1N1 16E6E7, H1N1 16E6E7m, parental influenza virus (delNS1), or HPV16 L1/L2-E7 VLP, and boosted either with PBS, the corresponding H3N2 influenza serotype, or VLPs 10 days later. Animals were monitored once per week and mean tumor volumes ± SEM of individual animals of one representative experiment of two are shown. Statistical significance of differences recorded for immunized groups and mock treated animals, or immunized groups and parental virus treated animals, were calculated by 1-way ANOVA and subsequent Dunnett’s multiple comparison test. Days after TC-1 tumor inoculation are indicated.